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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
26/09/2014 |
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Actualizado : |
18/03/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
IBÁÑEZ, F.; REZANO, A.; FREDES, A.; RODRIGUEZ, G.; REGGIO, A.; VILARÓ, F. |
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Afiliación : |
FACUNDO IBÁÑEZ SILVA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANABELA REZANO MATKOVICH, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; ALEJANDRO DAVID FREDES SIVOPLAS, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; GUSTAVO ADOLFO RODRIGUEZ TEXEIRA, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; ADRIANA MARIA REGGIO BIGANZOLI, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; FRANCISCO LUIS VILARO PAREJA, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
Development of a rapid microplate assay for determining pungency in onion breeding program. |
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Fecha de publicación : |
2012 |
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Fuente / Imprenta : |
Acta Horticulturae (ISHS), 2012, no. 935, p. 61-65. |
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ISSN : |
0567-7572 |
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DOI : |
10.17660/ActaHortic.2012.935.8 |
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Idioma : |
Inglés |
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Notas : |
XXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): International Symposium on New Developments in Plant Genetics and Breeding. |
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Contenido : |
ABSTRACT.
Onions with low levels of pungency and sweet flavor are becoming popular with consumers on an international level. The trend of greater demand in the international market will continue as sweet onions are introduced and promoted as a specialty item. Large-scale screening of onion bulbs for pungency requires a fast and cost-efficient method. The original method which was proposed by Schwimmer and Weston (1961), it is slow and tedious when large quantity of samples is analyzed in breeding programs. Moreover, it involves the use of dinitrophenylhydrazine, a chemical difficult to handle and very toxic. In our breeding program for developing low pungency cultivars, the screening is performed for more than 3000 bulbs annually. A 96-well microplate procedure for pyruvic acid analysis was developed in order to screen a large number of onion bulbs in our breeding rogram. The new method is based on the selective reaction of p-dimethylaminobenzaldehyde with enzymatically produced pyruvic acid. Analysis conditions were optimized with Response Surface Modelling using a Box-Behnken Design. With this method it is possible to determine the pyruvic acid content in 96 onion bulbs in just a few minutes. In a test using our cultivars, this method showed high correlations with spectrophotometric method (R2>0.95). |
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Thesagro : |
AJO. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 02138naa a2200229 a 4500
001 1050611
005 2022-03-18
008 2012 bl uuuu u00u1 u #d
022 $a0567-7572
024 7 $a10.17660/ActaHortic.2012.935.8$2DOI
100 1 $aIBÁÑEZ, F.
245 $aDevelopment of a rapid microplate assay for determining pungency in onion breeding program.$h[electronic resource]
260 $c2012
500 $aXXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): International Symposium on New Developments in Plant Genetics and Breeding.
520 $aABSTRACT. Onions with low levels of pungency and sweet flavor are becoming popular with consumers on an international level. The trend of greater demand in the international market will continue as sweet onions are introduced and promoted as a specialty item. Large-scale screening of onion bulbs for pungency requires a fast and cost-efficient method. The original method which was proposed by Schwimmer and Weston (1961), it is slow and tedious when large quantity of samples is analyzed in breeding programs. Moreover, it involves the use of dinitrophenylhydrazine, a chemical difficult to handle and very toxic. In our breeding program for developing low pungency cultivars, the screening is performed for more than 3000 bulbs annually. A 96-well microplate procedure for pyruvic acid analysis was developed in order to screen a large number of onion bulbs in our breeding rogram. The new method is based on the selective reaction of p-dimethylaminobenzaldehyde with enzymatically produced pyruvic acid. Analysis conditions were optimized with Response Surface Modelling using a Box-Behnken Design. With this method it is possible to determine the pyruvic acid content in 96 onion bulbs in just a few minutes. In a test using our cultivars, this method showed high correlations with spectrophotometric method (R2>0.95).
650 $aAJO
700 1 $aREZANO, A.
700 1 $aFREDES, A.
700 1 $aRODRIGUEZ, G.
700 1 $aREGGIO, A.
700 1 $aVILARÓ, F.
773 $tActa Horticulturae (ISHS), 2012, no. 935, p. 61-65.
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas; INIA Tacuarembó; INIA Treinta y Tres. |
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Fecha actual : |
05/10/2015 |
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Actualizado : |
30/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Nacionales |
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Circulación / Nivel : |
Nacional - B |
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Autor : |
UNIVERSIDAD DE LA REPÚBLICA (UDELAR)/ FACULTAD DE AGRONOMÍA; INIA (INSTITUTO NACIONAL DE INVESTIGACIÓN AGROPECUARIA) |
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Título : |
AGROCIENCIA. Revista Científica de la Facultad de Agronomía e INIA, v.19, Número Especial, 2015. Congreso CIGR. 3r. Inter-Regional CIGR Conference on Land and Water Challenges: Tools for developing "Dr. Mario García Petillo". |
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Fecha de publicación : |
2015 |
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Fuente / Imprenta : |
Montevideo (Uruguay): INIA, 2015. |
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Volumen : |
19, Special Issue |
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Páginas : |
85 p. |
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ISSN : |
1510-0839 |
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Idioma : |
Inglés |
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Notas : |
EDITORIAL BOARD SPECIAL ISSUE: García, C. (Instituto Nacional de Investigación Agropecuaria); Puppo, L. (Universidad de la República, Facultad de Agronomía); Tarjuelo, J.M. (Univ. Castilla-La Mancha, España); Carsjens, G-.J. (Univ.Wageningen, Holanda). |
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Contenido : |
This Special Issue of AGROCIENCIA Uruguay contains the conferences and a selection of papers presented during the 3rd InterRegional CIGR Conference that was held in La Estanzuela, Colonia, Uruguay, on September of 2015. |
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Thesagro : |
ANIMAL PRODUCTION; BOTANICA; BOTANY; CIENCIAS SOCIALES; GRAS; METEOROLOGIA; PLANT PROTECTION; PRODUCCIÓN ANIMAL; PROTECCIÓN DE LAS PLANTAS; SOCIAL SCIENCE; SOIL; SUELO. |
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Asunto categoría : |
A50 Investigación agraria |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/5026/1/Agrociencia-Congreso-CIGR2015.pdf
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Marc : |
LEADER 01529nam a2200313 a 4500
001 1053648
005 2019-10-30
008 2015 bl uuuu u01u1 u #d
022 $a1510-0839
100 1 $aUNIVERSIDAD DE LA REPÚBLICA (UDELAR)/ FACULTAD DE AGRONOMÍA
245 $aAGROCIENCIA. Revista Científica de la Facultad de Agronomía e INIA, v.19, Número Especial, 2015. Congreso CIGR. 3r. Inter-Regional CIGR Conference on Land and Water Challenges$bTools for developing "Dr. Mario García Petillo".
260 $aMontevideo (Uruguay): INIA$c2015
300 $a85 p. 19, Special Issue
490 $v19, Special Issue
500 $aEDITORIAL BOARD SPECIAL ISSUE: García, C. (Instituto Nacional de Investigación Agropecuaria); Puppo, L. (Universidad de la República, Facultad de Agronomía); Tarjuelo, J.M. (Univ. Castilla-La Mancha, España); Carsjens, G-.J. (Univ.Wageningen, Holanda).
520 $aThis Special Issue of AGROCIENCIA Uruguay contains the conferences and a selection of papers presented during the 3rd InterRegional CIGR Conference that was held in La Estanzuela, Colonia, Uruguay, on September of 2015.
650 $aANIMAL PRODUCTION
650 $aBOTANICA
650 $aBOTANY
650 $aCIENCIAS SOCIALES
650 $aGRAS
650 $aMETEOROLOGIA
650 $aPLANT PROTECTION
650 $aPRODUCCIÓN ANIMAL
650 $aPROTECCIÓN DE LAS PLANTAS
650 $aSOCIAL SCIENCE
650 $aSOIL
650 $aSUELO
700 1 $aINIA (INSTITUTO NACIONAL DE INVESTIGACIÓN AGROPECUARIA)
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INIA Tacuarembó (TBO) |
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